Research work of Biotechnology Laboratory

In this page you can find the extensive list of our research achievements, including publications in international peer-reviewed journals, patents, and chapters in books. These represent the culmination of our dedicated efforts to push the boundaries of biotechnology and make a meaningful impact in the field. Our team is proud to share our findings with the world and we hope that they will inspire future generations of biotechnology researchers. Browse through our list and see for yourself the breadth and depth of our work.

Chapters in Books

A. Patents

“A. Τzakos, H. Stamatis , I. Gerotanasis, A, Cjatzikonstantinou,M. Chatziathanasiadou System and method for the production, characterization and biological evaluation of natural product analogues” Hellenic Industrial Property Organisation OBI-GREECE 1009100.

B. Publications in International Peer Reviewed Journals


1) A. Xenakis, H. Stamatis, A. Malliaris, F.N. Kolisis. Effect of alcohols on the structure of AOT reverse micelles with respect to different enzyme activity. Progress in Colloid and Polymer Science, 93, 373-376, 1993.

2) H. Stamatis, A. Xenakis, M. Provelegiou F.N. Kolisis. Εsterification reactions catalyzed by lipases in microemulsions. The role of enzyme localiza­tion in relation to its selectivity. Biotechnology and Bioengineering, 42, 103-110, 1993.

3) H. Stamatis, F.N. Kolisis, A. Xenakis. Enantiomeric selectivity of a lipase from Penicillium simplicissimum in the esterification of menthol in microemulsions. Biotechnology Letters, 15 (5), 471-476, 1993.

4) H. Stamatis, A. Xenakis, U. Menge and F.N. Kolisis. Kinetic study of lipase catalyzed esterification reactions in microemulsions. Biotechnology and Bioengineering, 42, 931-937, 1993.

5) H. Stamatis, F.N. Kolisis, A. Xenakis, U. Bornscheuer,T. Scheper, U. Menge. Pseudomonas cepacia lipase: Esterification reactions in AOT microemulsion systems. Biotechnology Letters 15(7), 703-708, 1993.


6) U. Bornscheuer, H. Stamatis, A. Xenakis, T. Yamane, F.N. Kolisis. A comparison of different strate­gies for lipase-catalysed synthesis of partial glycerides. Biotechnology Letters 16, 697-702, 1994.

7) H. Stamatis, A. Xenakis, F.N. Kolisis, A. Malliaris. Lipase localization in w/o microemulsions studied by fluorescence energy transfer. Progress in Colloid and Polymer Science, 97, 253-255, 1994


8) H. Stamatis, A. Xenakis, E. Dimitriadis, F.N.Kolisis. Catalytic behavior of Pseudomonas cepacia lipase in w/o microemulsions. Biotechnology and Bioengineering, 45, 33-41, 1995.

9) H. Stamatis, A. Xenakis, F.N.Kolisis. Studies on enzyme reuse and product recovery in lipase catalyzed-reactions in microemulsions. Annals of the New York Academy of Science, 750, 237-241, 1995.

10) A. Ballesteros, U. Bornscheuer, A. Capewell, D. Combes, J-S. Condoret, K. Koening, F.N. Kolisis, A. Marty, U. Menge, T. Scheper, H. Stamatis. Enzymes in non-conventional phases. – Review article Biocatalysis and Biotransformations, 13, 1-42, 1995.


11) J.B. Macris, H. Stamatis and F.N. Kolisis. Microemulsions as a tool for the regioselective lipase-catalysed esterifica­tion of aliphatic diols. Applied Microbiology and Biotechnology. 46, 521-524, 1996.

12) V. Papadimitriou, A. Xenakis, C.T. Cazianis, H. Stamatis, M. Egmond. EPR studies of cutinase in microemulsions. Annals of the New York Academy of Science, 799, 275-280, 1996.

13) H. Stamatis, J. Macris and F.N. Kolisis. Esterification of hydrophilic diols catalysed by lipases in microemulsions. Biotechnology Letters, 18, 541-546, 1996.

14) S. Avramiotis, H. Stamatis, F.N. Kolisis, P. Lianos, A.Xenakis. Structural studies of lecithin and AOT-based water-in-oil microemulsions in the presence of lipase. Langmuir, 12, 6320-6328, 1996.



15) S. Avramiotis, H. Stamatis, F.N. Kolisis, A. Xenakis. Pseudomonas cepacia lipase localization in lecithin and AOT w/o microemulsions. A fluorescence energy transfer study. Progress in Colloid and Polymer Sci­ence, 105, 180-183, 1997.

16) V. Sereti, H. Stamatis, F.N. Kolisis. Improved stability and reactivity of Fusarium solani cutinase in supercritical CO2 Biotechnology Techniques 11, 661-665, 1997.



17) C. Tsitsimpikou, H. Stamatis, V. Sereti, H. Daflos and F.N. Kolisis. Acylation of glucose catalysed by lipases in supercritical carbon dioxide. Journal of Chemical Technology and Biotechnology, 71, 309-314, 1998.<309::AID-JCTB859>3.0.CO;2-L

18) H. Stamatis, P. Christakopoulos, D. Kekos, B.J. Makris, F.N. Kolisis. Studies on the synthesis of short-chain geranyl esters catalyzed by Fusarium oxysporum esterase in organic solvents. Journal of Molecular Catalysis B: Enzymatic, 4, 229-236, 1998.

19) P. Christakopoulos, B. Tzalas, D. Mamma, H. Stamatis, Liadakis G.N., C. Tzia, D. Kekos, F.N. Kolisis, B.J. Makris. Production of an esterase from Fusarium oxysporum catalysing transesterification reactions in organic solvents. Process Biochemistry, 33(5), 585-594, 1998.

20) H. Stamatis, V. Sereti and F.N. Kolisis. Studies on the enzymatic synthesis of sugar esters in organic medium and supercritical carbon dioxide. Chemical and Biochemical Engineering Quarterly, 12, 151-156, 1998.

21) V. Sereti, H. Stamatis, E. Koukios and F.N. Kolisis. Enzymatic acylation of cellulose acetate in organic media. Journal of Biotechnology, 66, 219-223, 1998.



22) H. Stamatis and A. Xenakis. Biocatalysis using microemulsion-based polymer gels containing immobilized lipase, Journal of Molecular Catalysis B: Enzymatic, 6, 399-406, 1999

23) Η. Stamatis, A. Xenakis, F.N. Kolisis. Bioorganic reactions in microemulsions: The case of lipase. Biotechnology Advances, 17(3), 293-318, 1999. (Invited paper)

24) A. Xenakis and H. Stamatis. Lipase immobilization on microemulsion-based polymer gels. Progress in Colloid and Polymer Science, 112, 132-135, 1999

25) H. Stamatis, V. Sereti and F.N. Kolisis. Studies on the enzymatic synthesis of lipophilic derivatives of natural antioxidants, Journal of the American Oil Chemist’s Society, 76(12), 1505-1510, 1999.

26) D.G. Hatzinikolaou, E. Kourentzi, H. Stamatis, P. Christakopoulos, F.N. Kolisis, D. Kekos and B.J. Makris. A novel lipolytic activity by Rhodotorula glutinis cells. Production, partial characterization and application in the synthesis of esters. Journal of Bioscience and Bioengineering, 88, 36-39, 1999.



27) H. Stamatis, E.C.Voutsas, C. Delimitsou, F.N. Kolisis and D. Tassios. Enzymatic production of alkyl esters through lipase-catalyzed transesterification reactions in organic solvents: Solvent effect and prediction of equilibrium conversions. Biocatalysis and Biotransformation, 18, 259-269, 2000.

28) A. Pastou, H. Stamatis and A. Xenakis. Microemulsion-based organogels containing lipase: Application in the synthesis of esters. Progress in Colloid and Polymer Science 115, 192-195, 2000



29) H. Stamatis, V. Sereti and F.N. Kolisis. Enzymatic synthesis of hydrophilic and hydrophobic derivatives of natural phenolic acids in organic media. Journal of Molecular Catalysis B: Enzymatic 11, 323-328, 2001

30) V. Sereti, H. Stamatis, C. Pappas, M. Polissiou and F.N. Kolisis. Enzymatic acylation of hydroxypropyl cellulose in organic media and determination of the ester formation by Diffuse Reflectance Infrared Fourier Transform (DRIFT) spectroscopy. Biotechnology and Bioengineering 72, 495-500, 2001<495::AID-BIT1012>3.0.CO;2-T

31) A. Kontogianni, V. Skouridou, V. Sereti, H. Stamatis and F.N. Kolisis. Regioselective acylation of flavonoids catalysed by lipase in non-toxic media. European Journal of Lipid Science and Technology, 103, 655-660, 2001.<655::AID-EJLT655>3.0.CO;2-X



32) E.C. Voutsas, H. Stamatis, F.N. Kolisis and D. Tassios. Solvent effect on equilibrium position and initial rate of lipase-catalyzed esterification reactions in organic solvents: Experimental results and prediction capabilities. Biocatalysis and Biotransformation, 20, 101-109, 2002.

33) Ch. Delimitsou, M. Zoumpanioti, A. Xenakis and H. Stamatis. Activity and stability studies of Mucor miehei lipase immobilized in novel microemulsion-based organogels. Biocatalysis and Biotransformation, 20, 319-327, 2002

34) E. Franqueville, H. Stamatis, H. Loutrari, A. Friboulet, F.N. Kolisis. Studies on the catalytic behavior of a cholinesterase-like abzyme in an AOT microemulsion system. Journal of Biotechnology, 97, 177-182, 2002.



35) P. Christakopoulos, P. Katapodis, E. Kalogeris, D. Kekos, B.J. Macris, H. Stamatis, H. Skaltsa. Antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases. International Journal of Biological Macromolecules 31, 171-175, 2003

36) E., Topakas, H. Stamatis, P. Biely , D. Kekos, B.J. Macris , P. Christakopoulos. Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures. Journal of Biotechnology, 102, 33-44, 2003

37) V. Skouridou, H. Stamatis and F.N. Kolisis. Use of essential oils as media for the enantioselective esterification of the monoterpene perillyl alcohol catalyzed by lipase. European Journal of Lipid Science and Technology, 105 (3-4), 115-120, 2003.

38) E. Topakas, H. Stamatis, M. Mastihubova, P. Biely, D. Kekos, B.J. Macris, P. Christakopoulos. Purification and characterization of a feruloyl esterase (FAE-I) from Fusarium oxysporum catalyzed transesterification of phenolic acid esters. Enzyme and Microbial Technology 33, 729-737, 2003.

39) V. Skouridou, H. Stamatis and F.N. Kolisis. Lipase-mediated epoxidation of α-pinene. Journal of Molecular Catalysis B: Enzymatic, 21, 67-69, 2003

40) Y. Sanakis, D. Mamma, P. Christakopoulos, H. Stamatis. Catechol 1,2-Dioxygenase from Pseudomonas putida in organic media – An Electron Paramagnetic Resonance study. International Journal of Biological Macromolecules 33, 101-106, 2003

41) A. Kontogianni, V. Skouridou, V. Sereti, H. Stamatis and F.N. Kolisis. Lipase-catalyzed esterification of rutin and naringin with fatty acids of medium carbon. Journal of Molecular Catalysis B: Enzymatic, 21, 59-62, 2003.

42) V. Skouridou, H. Stamatis and F.N. Kolisis. A study on the process of the lipase-catalyzed synthesis of α-pinene oxide in organic solvents. Biocatalysis and Biotransformation 21, 285-290, 2003

43) E. Franqueville, H. Loutrari, F. Mellou, H. Stamatis, A. Friboulet, F.N. Kolisis. Reverse micelles, a system for antibody-catalysed reactions. Journal of Molecular Catalysis B: Enzymatic 21 15-17, 2003



44) E. Topakas, H. Stamatis, P. Biely, P. Christakopoulos. Purification and characterization of a thermophilic feruloyl esterase from Sporotrichum thermophile (StFAE-A) catalyzing transesterification of phenolic acid esters. Applied Microbiology and Biotechnology 63(6), 686-690, 2004.

45) P. Dominguez de Maria, A. Xenakis, H. Stamatis, J. V. Sinisterra. Lipase Factor (LF) as a characterization parameter to explain the catalytic activity of crude lipases from Candida rugosa, ATCC 14830, free or immobilized in microemulsion based organogels. Enzyme and Microbial Technology 35 (4), 277-283, 2004.

46) V Skouridou, E.D. Chrysina, H. Stamatis, N. Oikonomakos, F. N. Kolisis. Kinetic and modelling studies on the lipase catalysed enantioselective esterification of (±)-perillyl alcohol. Journal of Molecular Catalysis B: Enzymatic, 29, 9-12, 2004.

47) P. Dominguez de Maria, A. Xenakis, H. Stamatis, J. V. Sinisterra. Unexpected reaction profile observed in the synthesis of propyl laurate when using Candida rugosa lipases immobilized in microemulsions based organogels. Biotechnology Letters, 26, 1517-1520, 2004.

48) M.Zoumpanioti, E.Karavas, C.Skopelitis, H.Stamatis, A. Xenakis. Lecithin organogels as model carriers of pharmaceuticals. Progress in Colloid and Polymer Science, 123, 199-202, 2004.



49) F. Mellou, D. Lazari, H. Skaltsa, A.D. Tselepis, F.N. Kolisis, H. Stamatis. Biocatalytic preparation of acylated derivatives of flavonoid glycosides enhances their antioxidant and antimicrobial activity. Journal of Biotechnology, 116, 295-304, 2005

50) E. Topakas, C. Vafiadi, H. Stamatis, P. Christakopoulos. Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis. Enzyme and Microbial Technology, 36, 729-736, 2005



51) M. Zoumpanioti, M. Karali, A. Xenakis, H. Stamatis. Lipase biocatalytic processes in surfactant free microemulsion – like ternary systems and related organogels. Enzyme and Microbial Technology, 39, 531-539, 2006

52) E. Kalogeris, J. Sanakis, D. Mamma, P. Christakopoulos, D. Kekos, H. Stamatis. Properties of catechol 1,2-dioxygenase from Pseudomonas putida immobilized in calcium alginate hydrogels. Enzyme and Microbial Technology, 39, 531-539, 2006

53) M.H. Katsoura, A.C. Polydera, L. Tsironis, A.D. Tselepis, H. Stamatis. Use of ionic liquids as media for the biocatalytic preparation of flavonoid derivatives with antioxidant potency. Journal of Biotechnology 123, 491-503, 2006

54) M. Zoumpanioti, H. Stamatis, V. Papadimitriou, A. Xenakis. Catalytic and spectroscopic studies of lipases in ternary hexane-1-propanol-water microemulsion-like systems. Colloids and Surfaces B: Biointerfaces 47, 1-9, 2006

55) F. Mellou, H. Loutrari, H. Stamatis, C. Roussos, F. N. Kolisis. Enzymatic esterification of flavonoids with unsaturated fatty acids. Effect of the novel esters on vascular endothelial growth factor release from K562 cells. Process Biochemistry 41, 2029-2034, 2006



56) M.H. Katsoura , A.C. Polydera, P. Katapodis, F.N. Kolisis, H. Stamatis. Effect of different reaction parameters on the lipase-catalyzed selective acylation of polyhydroxylated natural compounds in ionic liquids. Process Biochemistry 2007 42, 1326-1334, 2007



57) A. A. Tzialla, E. Kalogeris, D. Gournis, Y. Sanakis and H. Stamatis. Enhanced catalytic performance and stability of chloroperoxidase from Caldariomyces fumago in surfactant free ternary water-organic solvent systems. Journal of Molecular Catalysis B: Enzymatic 51 (1-2), pp. 24-35, 2008

58) E. Serefoglou, K. Litina, D. Gournis, E. Kalogeris, A.A Tzialla, H. Stamatis, E.Maccallini, M. Lubomska, P. Rudolf. Smectite clays as solid supports for immobilization of β-glucosidase: Synthesis, Characterization and Biochemical properties. Chemistry of Materials, 20 (12): 4106-4115, 2008

59) M. Zoumpanioti, P. Parmaklis, P. Dominguez de Maria, H. Stamatis, J.V. Sinisterra, A. Xenakis. Esterification reactions catalyzed by lipases immobilized in organogels. Effect of temperature and substrate diffusion. Biotechnology Letters 30 (9), pp. 1627-1631, 2008



60) E. Theodosiou, M. H. Katsoura, H. Loutrari, K. Purchartova, V. Kren, F.N. Kolisis, H. Stamatis. Enzymatic preparation of acylated derivatives of silybin in organic and ionic liquids media and evaluation of their anti-tumor proliferative activity. Biocatalysis and Biotransformations 27(3): 161-169, 2009

61) D. Mamma D. Hatzinikolaou D. Kekos, H. Stamatis E. Kalogeris. Adsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substrates. World J Microbiol Biotechnol. 25 (5), pp. 781-788, 2009

62) E. Xanthakis, S. Magkouta, H. Loutrari, H. Stamatis, C. Roussos, F.N. Kolisis. Enzymatic synthesis of perillyl alcohol derivatives and investigation of their antiproliferative growth activity. Biocatalysis and Biotransformation 27 (3), pp. 170-178, 2009

63) I.V. Pavlidis, D. Gournis, G.K. Papadopoulos, H. Stamatis. Lipases in Water-in-Ionic Liquid Microemulsions. Structural and Activity Studies Journal of Molecular Catalysis B: Enzymatic 60, 50–56, 2009

64) M. H. Katsoura, A. C. Polydera, L. D. Tsironis, M.P. Petraki, S. Kostić Rajačić, A. D. Tselepis H. Stamatis. Efficient enzymatic preparation of hydroxycinnamates in ionic liquids enhances their antioxidant effect on lipoproteins oxidative modification. New Biotechnology 26, 83-91, 2009

65) A. A Tzialla, A.A. Taha, E. Kalogeris, H. Stamatis. Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems. Biotechnology Letters, 31:1451–1456, 2009

66) A. A Tzialla, E. Kalogeris, A. Enotiadis, A.A. Taha, D. Gournis, H. Stamatis. Effective immobilization of Candida antarctica lipase B in organic-modified clays: Application for the epoxidation of terpenes. Materials Science and Engineering B 165 173–177, 2009



67) A.A Tzialla, I.V. Pavlidis M Felicissimo, P. Rudolf, D. Gournis, H. Stamatis. Lipase immobilization on smectite nanoclays: Characterization and application to the epoxidation of α-pinene. Bioresource Technology 101 1587–1594, 2010

68) M. Zoumpanioti, H. Stamatis. Microemulsion-based organogels as matrices for lipase immobilization. Biotechnology Advances 28, 395–406, 2010

69) E. Panteli, P. Saratsioti, H. Stamatis, E. Voutsas. “Solubilities of Cinnamic Acid Esters in Organic Solvents”. Journal of Chemical Engineering Data, 55 (2), pp. 745-749 2010.

70) E. Xanthakis, E. Theodosiou, S. Magkouta, H. Stamatis, H. Loutrari, C. Roussos, F.N Kolisis. Enzymatic Transformation of Flavonoids and Terpenoids: Structural and Functional Diversity of the Novel Derivatives. Pure and Applied Chemistry 82 (1), 1–16, 2010.

71) I.V. Pavlidis, T. Tsoufis, A. Enotiadis, D. Gournis, H. Stamatis. Functionalized Multi-Wall Carbon Nanotubes for Lipase Immobilization. Advanced Engineering Materials 12 (5), pp. B179-B183, 2010

72) Zoumpanioti M, Merianou E, Karandreas T, H. Stamatis, Xenakis A. Esterification of phenolic acids catalyzed by lipases immobilized in organogels. Biotechnology Letters 32 (10) 1457-1462

73) I.V. Pavlidis, K. Tzafestas and H. Stamatis. Water-in-ionic liquid microemulsions-based organogels as novel matrices for enzyme immobilization. Biotechnology Journal 5 (8), pp. 805-812, 2010



74) Τheodosiou Ε., Loutrari, Η., Stamatis Η., Roussos C. Kolisis F. Biocatalytic synthesis and antitumor activities of novel silybin acylated derivatives with dicarboxylic acids. New Biotechnology, 28 (4), pp. 342-348, 2011



75) E. Kyriakou, A. Primikyri, P. Charisiadis, M. Katsoura, I. P. Gerothanassis, H. Stamatis, A. G. Tzakos. Unexpected Enzyme-Catalyzed Regioselective Acylation of Flavonoid Aglycones and Rapid Product Screening. Organic Biomolecular Chemistry 10 (9), pp. 1739-1742,2012, DOI: 10.1039/C2OB06784F

76) A. Primikyri, E. Kyriakou, P. Charisiadis, C. Tsiafoulis, H. Stamatis A.G. Tzakos, I.P. Gerothanassis. Fine-Tuning the Diffusion Dimension of -OH Groups for the Application of High Resolution DOSY in Mixture Analysis of Organic Compounds Tetrahedron 68 (34) , pp. 6887-6891 , 2012

77) I.V. Pavlidis, T. Vorhaben, T. Tsoufis, P. Rudolf, U.T. Bornscheuer, D. Gournis and H. Stamatis. Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials. Bioresource Technology 115 , pp. 164-171, 2012

78) I.V. Pavlidis, T. Vorhaben, D. Gournis, G. K. Papadopoulos, U.T. Bornscheuer, and H. Stamatis. Regulation of catalytic behavior of hydrolases through interactions with functionalized carbon-based nanomaterials. Journal of Nanoparticle Research 14 (5), art. no. 0842, 2012



79) A.Taha, I. Shwaish, A.H Mohammed and H. Stamatis. Production of a laccase from Botrytis cinerea (DSMZ 877) and application for textile phenolic dye decolorization. Energy Procedia Journal 36, 862 – 871, 2013

80) A.A. Papadopoulou, M.H. Katsoura, A. Chatzikonstantinou, E. Kyriakou, A.C. Polydera, A.G.Tzakos, H. Stamatis. “Enzymatic hybridization of -lipoic acid with bioactive compounds in ionic solvents”. Bioresourse Technology 136, 41–48, 2013

81) M. Patila, I. Pavlidis, P. Katapodis, D. Gournis, H. Stamatis. Enhancement of cytochrome c catalytic behaviour by affecting the heme environment using functionalized carbon-based nanomaterials. Process Biochemistry 48, 1010–1017, 2013



82) E. Theodosiou, K. Purchartová, H. Stamatis, F. Kolisis, V. Křen. “Bioavailability of silymarin: Drug formulations and biotransformation“. Phytochemistry Reviews 13 (1), 1-18, 2014

83) I V. Pavlidis, M. Patila, U.Τ. Bornscheuer, D. Gournis, H. Stamatis. Graphene-based nanobiocatalytic systems: Recent advances and future prospects. Trends in Biotechnology 32 (6), 312-320, 2014 (Invited paper)



84) Elena Geromichalou, Nisar Sayyad, Eleni Kyriakou, Alexandra Chatzikonstantinou, Efstathia Giannopoulou, Haralabos P. Kalofonos, Haralambos Stamatis, Andreas G. Tzakos. Regioselective Chemical and Rapid Enzymatic Synthesis of a Novel Re-dox – Antiproliferative Molecular Hybrid”. European Journal of Medicinal Chemistry 96, 47-57, 2015

85) Ali A. Taha, Nada Husham Abdulateef, Ghassaq Tariq Sadiq, Mohammed Omar Abdullatif, Haider Abdullzahra Glaiym, Haralambos Stamatis and Batol Imran Dheeb. Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS), Egypt. Acad. J. Biolog. Sci., 7(1): 19 – 27 (2015)

86) Stelios Voulgaris, Athina A.Papadopoulou, Efthimia Alevizou, Haralambos Stamatis, Epaminondas Voutsas. Measurement and Prediction of Solvent Effect on Enzymatic Esterification Reactions Fluid Phase Equilibria 398, 51-62, 2015



87) A. A. Papadopoulou, A. Tzani, D. Alivertis, M.H. Katsoura, A.C. Polydera, A. Detsi and H. Stamatis. Hydroxyl ammonium ionic liquids as media for biocatalytic oxidations. Green Chemistry, 18, 1147–1158, 2016 DOI: 10.1039/C5GC02381E

88) M. Patila, I.V. Pavlidis, A. Kouloumpis, K. Dimos, K. Spyrou, P. Katapodis, D. Gournis, H. Stamatis. Graphene oxide derivatives with variable alkyl chain length and terminal functional groups as supports for stabilization of cytochrome c. International Journal of Biological Macromolecules, 84, 227–235, 2016.

89) A.Giannakas, M.Vlacha, C. Salmasa, A. Leontiou, P. Katapodis, H. Stamatis, N-M. Barkoula, A. Ladavos. Preparation, characterization, mechanical, barrier and antimicrobial properties of chitosan/PVOH/clay nanocomposites. Carbohydrate Polymers 140, 408–415, 2016

90) M. Vlacha, A. Giannakas, P. Katapodis, H. Stamatis, A. Ladavos, N-M. Barkoula. On the efficiency of oleic acid as plasticizer of chitosan/clay nanocomposites and its role on thermo-mechanical, barrier and antimicrobial properties–comparison with glycerol. Food Hydrocolloids 57, pp. 10-19, 2016

91) A.Xenakis, M. Zoumpanioti, H. Stamatis. Enzymatic reactions in structured surfactant-free microemulsions. Opinion in Colloid and Interface Science 22, pp. 41-45, 2016 (Invited paper)

92) M.Patila, A.Kouloumpis, D. Gournis, P. Rudolf, H.Stamatis. Laccase-functionalized graphene oxide assemblies as efficient nanobiocatalysts for oxidation reactions. Sensors, 2 16 (3), 287, 2016

93) Α. Papadopoulou, Ε. Efstathiadou, Μ., Patila, Α., Polydera, Η., Stamatis. Deep Eutectic Solvents as Media for Peroxidation Reactions Catalyzed by Heme-Dependent Biocatalysts. Industrial & Engineering Chemistry Research 55 (18), pp. 5145-5151, 2016



94) A Papadopoulou, A. Tzani, A.C Polydera, P. Katapodis, E. Voutsas, A. Detsi, H. Stamatis. Green biotransformations catalysed by enzyme-inorganic hybrid nanoflowers in environmentally friendly ionic solvents. Environmental Science and Pollution Research International 1-8, 2017 DOI:10.1007/s11356-017-9271-3

95) A.V. Chatzikonstantinou, G-F Norra, H. Stamatis, E. Voutsas. Prediction of Solvent Effect on Enzyme Enantioselectivity. Fluid Phase Equilibria 450, 126-132, 2017

96) K. Khoshnevisan, F.Vakhshiteh, M. Barkhi, H. Baharifar, E. Poor-Akbar, N. Zari, H. Stamatis, Al-K Bordba. Immobilization of cellulase enzyme onto magnetic nanoparticles: Applications and recent advances. Molecular Catalysis 442, 66-73, 2017



97) A. Chatzikonstantinou, M. Chatziathanasiadou, E. Ravera, M. Fragai, G. Parigi, I. Gerothanassis, C. Luchinat; H. Stamatis, A. Tzakos. Enriching the biological space of natural products, through real time biotransformation monitoring: the NMR tube bioreactor BBA – General Subjects 1862 (1), 1-8, 2018

98) P. Zygouri, T. Tsoufis, A. Kouloumpis, M. Patila, G. Potsi, A.A. Sevastos, Z. Sideratou, F. Katsaros, G. Charalambopoulou, H. Stamatis, P. Rudolf, T. A. Steriotis D. Gournis. Synthesis, characterization and assessment of hydrophilic oxidized carbon nanodiscs in bio-related applications. RSC Advances, 8 (1), 122-131 2018 DOI: 10.1039/C7RA11045F

99) G. Orfanakis, M. Patila, A.V. Chatzikonstantinou, K-M. Lyra, A. Kouloumpis, P. Katapodis, K. Spyrou, A. Paipetis, P. Rudolf, D. Gournis, H. Stamatis. Hybrid nanomaterials of magnetic iron nanoparticles and graphene oxide as matrices for the immobilization of ß-glucosidase: Synthesis, characterization and biocatalytic properties. Frontiers in Materials, April 2018 | Volume 5 | Article 25, 1-15, 2018

100) M. Patila, E.K. Diamanti, D. Bergouni, A.C. Polydera, D. Gournis, H. Stamatis. “Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c”. Nanomedicine Research Journal 3 (1), 10-18, 2018

101) AV. Chatzikonstantinou, E. Gkantzou, D. Gournis, M. Patila, H. Stamatis. Stabilization of laccase through immobilization on functionalized GO-derivatives. Methods in Enzymology 48-80, 609, 2018 (Invited paper)

102) E. Gkantzou, M. Patila, H. Stamatis. Magnetic microreactors with immobilized enzymes – from assemblage to contemporary applications. Catalysts, 8, 282; 1-16, 2018 (Invited paper)

103) E. Mitsou, E. P Kalogianni, D. Georgiou, H. Stamatis, A. Xenakis, M. Zoumpanioti. Formulation and Structural Study of a Biocompatible Water-in-Oil Microemulsion as an Appropriate Enzyme Carrier: The Model Case of Horseradish Peroxidase. Langmuir, 35, 150-160, 2018

104) P. Zygouri, T. Tsoufis, A. Kouloumpis, M. Patila, G. Potsi, Α. A. Sevastos, Z. Sideratou, F. Katsaros, G. Charalambopoulou, H. Stamatis, P. Rudolf, T. A. Steriotis, D. Gournis. Synthesis, characterization and assessment of hydrophilic oxidized carbon nanodiscs in biorelated applications. RSC Adv., 2018, 8, 122–131 DOI: 10.1039/C7RA11045F



105) D. Karageorgou, E. Thomou, N. T. Vourvou, K-M Lyra, N. Chalmpes, A. Enotiadis, K. Spyrou, P. Katapodis, D. Gournis, H. Stamatis. Antibacterial and Algicidal Effects of Porous Carbon Cuboid 2 Nanoparticles. ACS Omega 4 (3), 4991-5001 2019

106) R. Fotiadou, M, Patila, M. Amen Hammami, A. Enotiadis, D. Moschovas, K. Tsirka, K. Spyrou, E. P. Giannelis, A. Avgeropoulos, A. Paipetis, D. Gournis, H. Stamatis. Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations. Nanomaterials 9 (6), 808, 2019 DOI: 10.3390/nano9060808 (Invited paper)

107) AV Chatzikonstantinou, E Gkantzou, E Thomou, N Chalmpes, KM Lyra, VG Kontogianni, K.Spyrou, M. Patila, D. Gournis, H. Stamatis. Nanomaterials 9 (8), 1166, 2019 (Invited paper)

108) A Papadopoulou, D Zarafeta, AP Galanopoulou, H Stamatis. Enhanced Catalytic Performance of Trichoderma reesei Cellulase Immobilized on Magnetic Hierarchical Porous Carbon Nanoparticles. The protein journal 38 (6), 640-648, 2019



109) A. Giannakopoulou, E. Gkantzou, Α. Polydera and H. Stamatis. Multienzymatic Nanoassemblies: Recent Progress and Applications. Trends in Biotechnology 38 (2), 202-216, 2020 (Invited paper)

110) O. Boura-Theodoridou, A. Giannakas, P. Katapodis, H. Stamatis, A. Ladavos, N-M. Barkoula. Performance of ZnO/chitosan nanocomposite films for antimicrobial packaging applications as a function of NaOH treatment and glycerol/PVOH blending. Food Packaging and Shelf Life 23, 100456

111) M Patila, N Chalmpes, E Dounousi, H Stamatis, D Gournis. Use of functionalized carbon nanotubes for the development of robust nanobiocatalysts. Methods in Enzymology 630, 263-301 (Invited paper, in press)

112) A.V Chatzikonstantinou, A.C. Polydera, E.Thomou, N. Chalmpes, T. N Baroud, A. Enotiadis, L. Estevez, M. Patila, M. A. Hammami, K. Spyrou, E. P. Giannelis, A. G. Tzakos, D. Gournis, H. Stamatis. Lipase immobilized on magnetic hierarchically porous carbon materials as a versatile tool for the synthesis of bioactive quercetin derivatives. Bioresource Technology Reports 9, 100372, 2020

113) P. Koutsogiannis, E. Thomou, H. Stamatis, D. Gournis, P. Rudolf .Advances in Fluorescent Carbon Dots for Biomedical Applications. Advances in Physics 2020 (in press DOI: 10.1080/23746149.2020.1758592)

114) P. Zygouri, K. Spyrou, E. Mitsari, M. Barrio, R. Macovez, M. Patila, H. Stamatis. I. I.Verginadis, A.P.Velalopoulou, A. M. Evangelou, Z. Sideratou, D. Gournis, P. Rudolf. A facile approach to hydrophilic oxidized fullerenes and their derivatives as cytotoxic agents and supports for nanobiocatalytic systems. 2020 (in press) Scientific Report, DOI : 10.1038/s41598-020-65117-7

115) A.V Chatzikonstantinou, A.D. Tsiailanis, I.P Gerothanassis, H. Stamatis, E. Ravera, M. Fragai, C. Luchinat, G. Parigi, A. G Tzakos. The NMR tube bioreactor. Methods in Enzymology 633, 71-101, 2020


116) Hiba M Abdulhasan Alkafaji, Wasnaa H Mohammed, Zahraa A Sharba, Ali A Taha, Mohanad K Aneed, Mohammed M Farhan, Mohammed J Kazim, Manar M Rashid, Rafah A Salih, Noor S Yones, Rana S Noori, Nomaira G Abdulkareem, Haralambos Stamatis. TAT–Poly A1 peptides and siRNA loaded on calcium phosphate nanoparticles for ESR1 gene targeting in RD–cell line. Annals of Tropical Medicine and Health , 23, 232-128. 2020


117) Y. V Simos, K. Spyrou, M. Patila, N. Karouta, H. Stamatis, D. Gournis, E. Dounousi, D. Peschos. Trends of nanotechnology in type 2 diabetes mellitus treatment. Asian Journal of Pharmaceutical Sciences

118) N. Chalmpes, D. Moschovas, I. Tantis, A. B. Bourlinos, A. Bakandritsos , R. Fotiadou, M. Patila, H. Stamatis, A. Avgeropoulos , M.A. Karakassides, D.Gournis. Carbon Nanostructures Derived through Hypergolic Reaction of Conductive Polymers with Fuming Nitric Acid at Ambient Conditions. Molecules 2021, 26, 1595. 

119) R. Fotiadou, A.V Chatzikonstantinou, M. Amen Hammami, N.Chalmpes, D. Moschovas, K. Spyrou,  A. C Polydera, A. Avgeropoulos, D. Gournis, H. Stamatis. Green Synthesized Magnetic Nanoparticles as Effective Nanosupport for the Immobilization of Lipase: Application for the Synthesis of Lipophenols. Nanomaterials 2021, 11(2), 458; 

120) A. Kouloumpis, A. V Chatzikonstantinou, N. Chalmpes, T. Giousis, G. Potsi, P. Katapodis, H. Stamatis, D. Gournis, P. Rudolf. ACS Applied Nano Materials 4(3), 2333-2338, 2021 

121) E. Gkantzou, A.V Chatzikonstantinou, R. Fotiadou, A. Giannakopoulou, M. Patila, H. Stamatis. Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations. Biotechnology Advances, Volume 51, 107738, 2021 (Invited paper)

122) E. Gkantzou, K. Govatsi, A. V. Chatzikonstantinou, S.N. Yannopoulos, and H.Stamatis. Development of a ZnO Nanowire Continuous Flow Microreactor with β-Glucosidase Activity: Characterization and Application for the Glycosylation of Natural Products (Invited paper). ACS Sustainable Chem. Eng.2021,

123) M.G. Savvidou, M.M. Dardavila, I. Georgiopoulou, V. Louli, H. Stamatis, D.  Kekos, E. Voutsas. Optimization of microalga Chlorella vulgaris magnetic harvesting. Nanomaterials 11(6), 1614; 2021,

124) A. Giannakopoulou, A. V. Chatzikonstantinou, N. Chalmpes, G. Tsapara, D. Gournis, A. C. Polydera, H. Stamatis. Development of a novel bi-enzymatic nanobiocatalyst for the efficient bioconversion of oleuropein to hydroxytyrosol. Catalysts 1(6), 749-757, 2021 (Invited paper)


125) G. Bakratsas, A. Polydera, P. Katapodis, H. Stamatis. Recent trends in submerged cultivation of mushrooms and their application as a source of nutraceuticals and food additives. Future Foods, Volume 4, December 2021, 100086


126) A.V. Chatzikonstantinou, A. Giannakopoulou, S. Spyrou, Y. V Simos, V. G Kontogianni, D. Peschos, P. Katapodis, A. C Polydera, H. Stamatis. Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using immobilized enzyme reactors. Environmental Science and Pollution Research, 29, pages29624–29637, 2022

127) M.Patila, P.E. Athanasiou, L. Kortessis, G. Potsi, A. Kouloumpis, D. Gournis, H. Stamatis. Immobilization of Laccase on Hybrid Super-Structured Nanomaterials for the Decolorization of Phenolic Dyes. Processes 0(2), 233, 2022;

128) S. Asimakoula, A. Giannakopoulou, E. Lappa, E. Tsagogiannis, A. Primikyri, H. Stamatis, A-I.  Koukkou. Characterization of Gentisate 1,2-Dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and Its Stabilization by Immobilization on Nickel-Functionalized Magnetic Nanoparticles.  Appl. Microbiol. 2(1), 113-132, 2022;

129) N.A. Theocharidi, I. Balta, D. Houhoula, A. G Tsantes, G. P Lalliotis, A. C Polydera, H. Stamatis, P. Halvatsiotis. High Prevalence of Klebsiella pneumoniae in Greek Meat Products: Detection of Virulence and Antimicrobial Resistance Genes by Molecular Techniques. Foods 11(5), 708-714, 2022

130) E. Gkantzou, A. Skonta, A.Tsakni, A.Polydera, D. Moschovas, K. Spyrou, A. Avgeropoulos, D. Gournis, D. Houhoula, H. Stamatis. 3D printed PLA enzyme microreactors: Characterization and application for the modification of bioactive compounds. Journl of Biotechnology 350,75-85, 2022 2022

131) N. Chalmpes, M. Patila, A. Kouloumpis, C. Alatzoglou, K. Spyrou, M. Subrati, A. C Polydera, A.B Bourlinos, H. Stamatis, D. Gournis. Graphene Oxide–Cytochrome c Multilayered Structures for Biocatalytic Applications: Decrypting the Role of Surfactant in Langmuir–Schaefer Layer Deposition. ACS Appl. Mater. Interfaces 26204–26215, 14, 22, 2022.

132) P Zygouri, K Spyrou, DK Papayannis, G Asimakopoulos, E Dounousi, H. Stamatis, D. gournis, P. Rudolf. Comparative Study of Various Graphene Oxide Structures as Efficient Drug Release Systems for Ibuprofen. AppliedChem 2022, 2(2), 93-105;

133) E. Boli, N. Prinos, V. Louli, G. Pappa, H. Stamatis, K. Magoulas, E. Voutsas. Recovery of Bioactive Extracts from Olive Leaves Using Conventional and Microwave-Assisted Extraction with Classical and Deep Eutectic Solvents Separations. 2022, 9(9), 255;

134) A. Giannakopoulou, G. Tsapara, A. N Troganis, P. Koralli, Ch. L Chochos, A. C Polydera, P. Katapodis, N.-M Barkoula, H Stamatis. Development of a Multi-Enzymatic Approach for the Modification of Biopolymers with Ferulic Acid. Biomolecules 2022, 12(7), 992;

135) R. Fotiadou, M.G. Bellou, K. Spyrou, F. Yan, P. Rudolf, D. Gournis, H. Stamatis. Effect of deep eutectic solvents on the biocatalytic properties of β-glucosidase@ ZnOFe nano-biocatalyst. Sustainable Chemistry and Pharmacy 30, 100886, 2022

136) E. Papanikolaou, Y.V. Simos, K. Spyrou, E.I. Tzianni, P. Vezyraki, K. Tsamis, M.  Patila, S. Tigas, M.I Prodromidis, D. Gournis, H. Stamatis, D. Peschos, E. Dounousi. Is graphene the rock upon which new era continuous glucose monitors could be built? Experimental Biology and
Medicine, 2022

137) M.G. Bellou, E Gkantzou, A Skonta, D Moschovas, K Spyrou, A. Avgeropoulos, D. Gournis, H. Stamatis. Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media. Micromachines 2022, 13(11), 1954;

138) D. Karageorgou, I. Sainis, A. Touka, K. Vareli, H. Stamatis, P. Katapodis. Biomass and β-Glucosidase Production by the Cyanobacterium Pseudanabaena sp. under Heterotrophic Conditions. Biomass 2022, 2(4), 299-315;

139) A. Skonta, E. Gkantzou, K. Spyrou, S. Spyrou, A. Polydera, D. Gournis, H. Stamatis. 3DPrinted Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase. Catalysis Research 2022; 2(4); 032; doi:10.21926/cr.2204032.

C. Chapters in Books and Book Series (International Editions)

B1) H. Stamatis, A. Xenakis, H. Sztajer, U. Menge and F.N. Kolisis. Studies on the specificity of Penicillium simplicissimum lipase catalyzed reactions in microemulsions. Progress in Biotechnology. Biocatalysis in Non-Conventional Media (Tramper et al. eds), Amsterdam, Elsevier, Vol. 8, pp.733-738, 1992, ΙSBN 0-444-89046-7.

B2). V. Sereti, H. Stamatis, F.N. Kolisis. Studies on the regioselective acylation of sugars catalyzed by lipase in tert-butanol. Progress in Biotechnology. (Ballesteros et al. Eds), Amsterdam Elsevier, Vol 15, pp.725-730, 1998 ΙSBN 0-444-50519-9.

B3). V. Sereti, H. Stamatis, F.N. Kolisis. Strategies for the lipase-catalyzed regioselective acylation of polyols in organic media. Lipases and Lipids: Structure Function and Biotechnological Applications (G. Kokotos eds.), Cretan University Press, pp. 349-364, 2000 ISBN 960-524-111-0

B4) H. Stamatis, A. Xenakis and F.N. Kolisis. Synthesis of esters catalyzed by lipases in w/o microemulsions In: Methods in Biotechnology, Εnzymes in Nonaqueous Solvents, (Vulfson et al. Eds), Vol. 15, Chapter 28, Humana Press Inc. Totowa, NJ, pp. 331-338, 2001. ΙSBN 0-89603-929-3.

B5) H. Stamatis, V. Sereti and F.N. Kolisis. Lipase catalyzed synthesis of sugar fatty acid esters in supercritical carbon dioxide. In: Methods in Biotechnology, Εnzymes in Nonaqueous Solvents (Vulfson et al. Eds), Vol. 15, Chapter 39, Humana Press Inc, pp. 517-522, 2001, ΙSBN 0-89603-929-3.

B6) M. H. Katsoura, E. Theodosiou, H. Stamatis F. N. Kolisis. Strategies for the Biocatalytic Lipophilization of Phenolic Antioxidants In Modern biocatalysis, (Eds W-D Fessner, T Anthosen) Wiley 2009 pp. 323-333 ISBN: 978-3-527-62384-6

B7) A. Xenakis, V. Papadimitriou, H. Stamatis, F. N. Kolisis. “Biocatalysis in microemulsuions” in “Microemulsions: Properties and Applications” Surfactant Sci.Ser., 2009, Vol.144, pp.349-385. Ed. M.Fanun., CRC Press, Jerusalem, Israel ISBN 9780367386214

B8) H. Stamatis. Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, 7 Volume Set Michael C. Flickinger (Editor) Wiley-VCH Verlag 2010, 44 pages ISBN: 978-0-471-79930-6

B9) I.V. Pavlidis, A.A. Tzialla, A.Enotiadis, D. Gournis, H. Stamatis. Enzyme immobilization οn layered and nanostructured materials Ιn Biocatalysis in Polymer Chemistry, Edited by Katja Loos, Wiley-VCH Verlag & Co. KGaA, pp 35-64, 2010 ISBN: 978-3-527-32618-1 (Invited book chapter)

B10) I.V. Pavlidis, M. Patila, A. Polydera, D. Gournis, H. Stamatis. Immobilisation of enzymes and other biomolecules In Functionalization of Graphene Editor: Vasilios Georgakilas, Wiley-VCH Verlag & Co, 2014, 35 pages DOI:10.1002/9783527672790 (Invited book chapter)

B11) Μ. Katsoura, A. Papadopoulou A. Polydera, H. Stamatis. Effect of ionic liquids on catalytic properties and structure of biocatalysts In Ionic Liquid-Based Surfactant Science: Formulation, Characterization, and Applications edited by Bidyut K. Paul and Satya P. Mouli Wiley-VCH Verlag & Co, 2015 ISBN: 978-1-118-83419-0 (Invited book chapter)

B12) M. Patila, G. Orfanakis, A.C. Polydera, I.V. Pavlidis and H. Stamatis. “Graphene-based Nanobiocatalytic Systems”. Biocatalysis and Nanotechnology PanStanford Publishing, Singapore 2017 DOI: 10.4032/9781315196602 (Invited book chapter)

B13) E. M. Papamichael, H. Stamatis, P-Y. Stergiou, A. Foukis, O. A. Gkini. Advances in Enzyme Technology Pages 71-104, 2019

B14) E.Gkantzou, A. Skonta, A.-G Vasios, H. Stamatis. 3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization Methods in Molecular Biology: Multienzymatic Assemblies, pp 163–175 DOI: 10.1007/978-1-0716-2269-8_10

B15) A. Giannakopoulou, A. V Chatzikonstantinou, A. Tsakni, Ch. L Chochos, D.  Houhoula, H. Stamatis. Methods in Molecular Biology: Multienzymatic Assemblies, pp 279-296 DOI: 10.1007/978-1-0716-2269-8_17


B16) A. Giannakopoulou, M. Patila, N. Chalmpes, A. C Polydera, D. Gournis, H. Stamatis. A Four-enzyme Nanoassembly Consisting of Hydrolases and Oxidoreductases for Multi-step Cascade Reactions. Methods in Molecular Biology: Multienzymatic Assemblies, pp 263-278 DOI: 10.1007/978-1-0716-2269-8_16